METLA Vegetative propagation laboratory
Type of facility: Laboratory and greenhouse
Keywords: micropropagation, forest regeneration material, organogenesis, ornamental trees, rooted cuttings, somatic embryogenesis, tissue culture, tree breeding, selection, vegetative propagation, forest reproductive material, forest trees, tree breeding, tree selection
Who would find this useful? Forest research community, horticultural research community, plant science research community, tree plant producers/nurseries, tree breeders
"This is an uncomplicated, easy access to a skilful laboratory to learn specific scientific techniques and methods."
Spruce shoot cuttings being rooted. Photo: Teijo Nikkanen.
About the facility: The Metla Punkaharju Research Unit provides a complete infrastructure for vegetative propagation studies of trees. Within the facility, development and testing of different kinds of tissue culture techniques can be done, followed by acclimatisation and cultivation of the produced plants under greenhouse and nursery conditions. Experiments in grafting or rooting of cuttings can also be performed.
The infrastructure has a fully-equipped tissue culture and molecular biology laboratory (434 m2), to which a quality system to SFS-EN ISO/IEC 17025 standard is applied. The facility also includes special greenhouses for growing genetically modified plants as well as conventional greenhouses (including both all-year-round greenhouses and summer greenhouses), as well as a nursery.
Users will gain experience in working with different tools for vegetative propagation of conifers and/or broadleaved tree species, and comparison of different methods for their own use. The minimum output is a set of data (raw data) on the studied topic; analysed data are more typical. In addition to data sets, typically also either tissue cultured clones or regenerated plants are produced. Research reports, or in some cases even a manuscript for submission to international scientific journals, are possible.
- A guest can arrive with his/her own biological explant materials to be used in propagation studies. Alternatively, the EVOLTREE Punkaharju ISS Infrastructures can be utilised as an explant source.
- During the stay, the guest performs, for example, tissue culture initiation, proliferation/ multiplication, SE maturation, plantlet rooting / SE germination, or clonal identification experiments, supported by the guidance of the host scientist and technicians.
Results are collected in or converted to computer files. Data analysis and interpretation are based on in-house experience with software available. If possible, reports and manuscripts will be prepared while the guest is still at the site, or by email exchange afterwards.
Location: Punkaharju, Finland